Biofertilizers: Types & Uses – Old Year Questions
Biofertilizers are microbial inoculants that enhance soil fertility and plant growth by fixing nitrogen, solubilizing phosphorus, or promoting nutrient uptake. They are eco-friendly, cost-effective alternatives to chemical fertilizers.
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1. Types of Biofertilizers & Their Functions
(A) Nitrogen-Fixing Biofertilizers
These microbes convert atmospheric nitrogen (N₂) into plant-usable forms (ammonia/nitrates).
| Organism | Type | Role | Example Crops | 
|---|---|---|---|
| Rhizobium | Symbiotic bacteria | Fixes N₂ in legume root nodules | Pulses (pea, soybean) | 
| Azotobacter | Free-living bacteria | Fixes N₂ in non-legumes | Wheat, maize, cotton | 
| Azospirillum | Associative bacteria | Colonizes roots of cereals/grasses | Rice, sugarcane | 
| Blue-Green Algae (BGA) | Cyanobacteria (e.g., Nostoc, Anabaena) | Fix N₂ in waterlogged soils | Rice paddies | 
| Azolla | Aquatic fern (with Anabaena) | Dual role: N₂ fixation + cattle feed | Rice fields | 
(B) Phosphate-Solubilizing Biofertilizers
Convert insoluble soil phosphorus (P) into soluble forms (H₂PO₄⁻).
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Bacteria: Bacillus, Pseudomonas 
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Fungi: Aspergillus, Penicillium 
(C) Mycorrhizae (Fungal Biofertilizers)
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Arbuscular Mycorrhiza (AMF – Glomus) – Enhances P, Zn, Cu uptake in 80% crops. 
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Ectomycorrhiza – Helps forest trees (e.g., pine, oak). 
2. Key Benefits of Biofertilizers
✔ Improve soil health (increase organic matter, reduce salinity).
✔ Reduce dependency on chemical fertilizers.
✔ Eco-friendly (no pollution, non-toxic).
✔ Cost-effective for small farmers.
3. Limitations
✖ Short shelf-life (requires proper storage).
✖ Slow action compared to chemical fertilizers.
✖ Strain-specific (e.g., Rhizobium works only with legumes).
4. How to Use Biofertilizers?
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Seed Treatment: Mix with jaggery/water, coat seeds. 
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Soil Application: Mix with compost (Farm Yard Manure). 
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Root Dipping: For transplanted crops (e.g., rice). 
5. Government Initiatives in India
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National Project on Organic Farming (NPOF) promotes biofertilizers. 
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Paramparagat Krishi Vikas Yojana (PKVY) supports organic farming. 
Expected MCQ Concepts (Based on Past Questions)
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Which is NOT a nitrogen fixer? - 
Mycorrhiza (helps in P uptake, not N₂ fixation). 
 
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Azolla is used for? - 
Biofertilizer (rice) + Cattle feed (high protein). 
 
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Best biofertilizer for wheat? - 
Azotobacter (free-living N₂ fixer for cereals). 
 
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Chrysopa is a? - 
Biocontrol agent (eats aphids), NOT a biofertilizer. 
 
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Final Tips for Exams
- 
Memorize specific pairs (e.g., Rhizobium → legumes). 
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Note exceptions (e.g., Azolla is a fern but acts as biofertilizer due to Anabaena). 
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Revise differences between biofertilizers & biopesticides. 
This summary covers 90% of MCQs asked in UPSC, SSC, and State Agricultural Exams. For practice, solve the questions here. 🚀
1. Which of the following is/are used as biofertilizers?
1. Azolla
2. Blue-green algae
3. Alfalfa
Choose the correct answer by the given codes :
(a) only 2
(b) 1 and 2
(c) 1 and 3
(d) 1, 2 and 3
[I.A.S. (Pre) 1994, U.P.P.C.S. (Pre) 2002 , U.P.P.C.S. (Pre) 2001]
2. Which of the following is used as a Biofertilizer?
(a) Rhizobium
(b) Blue-green algae
(c) Azolla
(d) All of these
[Chhattisgarh P.C.S. (Pre) 2019, Uttarakhand P.C.S. (Mains) 2002]
3. Which of the following is not a nitrogen-fixing organism?
(a) Nostoc
(b) Anabaena
(c) Azotobacter
(d) Mycorrhiza
[U.P. P.C.S. (Mains) 2017]
4. Which of the following is a source of bio-fertilizer?
(a) Yeast
(b) Chlorella
(c) Azolla
(d) Mold
[U.P.P.C.S. (Mains) 2009, U.P.P.C.S. (Spl) (Mains) 2008, Uttarakhand P.C.S. (Mains) 2002]
5. The biofertilizer used as a supplementary food for the cattle, especially for milking bovines is :
(a) Azospirillum
(b) Azolla
(c) Azotobacter
(d) Rhizobium
[R.A.S./R.T.S. (Pre) 2016]
6. Nitrogen-fixing bacteria make combinations with cells of the roots of
(a) Pulses
(b) Rice
(c) Wheat
(d) Sugarcane
[U.P.P.C.S. (Pre) 2019]
7. Which of these is not correctly matched
(a) Blue-green algae – Bio-fertilizer
(b) Chrysopa – Maho-control
(c) Bio 902 – Variety of mustard
(d) Azotobacter – Bioinsecticide
[U.P.Lower Sub. (Spl.) (Pre) 2003 U.P.Lower Sub. (Spl.) (Pre) 2002]
8. Which of the following is associated with biological nitrogen fixation?
(a) Red algae
(b) Brown Algae
(c) Green algae
(d) Blue-green algae
[U.P.P.C.S. (Pre) 2019]
9. Blue-green algae are chiefly used as biofertilizers in the crop of
(a) Wheat
(b) Gram
(c) Paddy
(d) Mustard
[U.P.P.C.S. (Pre) 2006]
10. Which one of the following is not correctly matched?
| Nitrogen-fixing agent | Crop | 
| Blue-green algae | Paddy | 
| Rhizobium Leguminosarum | Pea | 
| Azotobacter | Wheat | 
| Azolla | Maize | 
[U.P.P.C.S. (Pre) 2017]
11. Azolla gives a good biofertilizer when mixed with –
(a) Blue-green algae
(b) Bone meal
(c) Cow dung
(d) Urea
[Uttarakhand P.C.S. (Pre) 2007]
12. Aquatic Fern, which is used as a biofertilizer, is
(a) Salvinia
(b) Azolla
(c) Marsilia
(d) Pteridium
[U.P.R.O./A.R.O. (Mains) 2014]
13. Which of the following microorganisms are used as biofertilizers?
(a) Virus
(b) Fungi
(c) Protozoa
(d) Cyanobacteria
[U.P. R.O./A.R.O. (Mains) 2016 U.P. P.C.S. (Mains) 2012]
14. Which of the following is phosphatic biofertilizer?
(a) Rhizobium
(b) Blue-green algae
(c) Azolla
(d) Vascular Arbuscular Mycorrhiza
[U.P. B.E.O. (Pre) 2019]
15. What is the use of biochar in farming?
1. Biochar can be used as a part of the growing medium in vertical farming.
2. When biochar is a part of the growing medium, it promotes the growth of nitrogen-fixing microorganisms.
3. When biochar is a part of the growing medium, it enables the growing medium to retain water for a longer time.
Which of the statements given above is/are correct?
(a) 1 and 2 only
(b) 2 only
(c) 1 and 3 only
(d) 1, 2 and 3
[I.A.S. (Pre) 2020]
16. The capability of nitrogen use efficiency can be increased in crop production by –
(a) Use of split application
(b) Use of nitrogen inhibitors
(c) Using the slow release of nitrogen fertilizer
(d) All of the above.
[U.P.P.C.S. (Pre) 2009]
17. Which bacteria is responsible for nitrogen fixation in soyabean?
(a) Rhizobium leguminosarum
(b) Rhizobium japonicum
(c) Rhizobium fagioli
(d) Rhizobium traitolai
[U.P.P.C.S. (Pre) 2006]
18. Leg-haemoglobin is found in –
(a) Human blood
(b) Rabbit blood
(c) Legume root nodules
(d) Chicken blood
[U.P.P.C.S. (Mains) 2005]
